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pproex hotub1 wang  (Addgene inc)


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    Structured Review

    Addgene inc pproex hotub1 wang
    Pproex Hotub1 Wang, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/popink+votu/pOPINK-vOTU+(CCHFV+OTU%2C+aa+1-183)+(Plasmid+%2361589)/pm41167191-489-254-259
    Average 93 stars, based on 6 article reviews
    pproex hotub1 wang - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability
    Article Snippet: .. A plasmid expressing GST-Neh2Dual in bacteria under control of the T7 promoter was constructed by replacing vOTU from pOPINK-vOTU (Addgene plasmid # 61589, a gift from David Komander ) using Gibson cloning. ..

    Article Title: Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability
    Article Snippet: .. A plasmid expressing GST-Neh2Dual in bacteria under control of the T7 promoter was constructed by replacing vOTU from pOPINK-vOTU (Addgene plasmid # 61589, a gift from David Komander ) using Gibson cloning. ..

    Article Title: Site-specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signaling.
    Article Snippet: .. Sequences of all the plasmids generated for this study were confirmed by Sanger sequencing. pcDNA3-Myc-human HOIP and pcDNA3-human-HOIL1L-HA were from Kazuhiro Iwai (Tokunaga et al, 2009), pGex6P1-human UbcH7 was from Katrin Rittinger (Stieglitz et al, 2012). pOPINK-vOTU (CCHFV OTU, aa1–183) (Addgene plasmid #61589) (Akutsu et al, 2011) and pOPINS-USP21 (USP, aa 196–565) (Addgene plasmid #61585) (Ye et al, 2011) were gifts from David Komander. ..

    Article Title: Site-specific ubiquitination of the E3 ligase HOIP regulates cell death and immune signaling
    Article Snippet: .. Sequences of all the plasmids generated for this study were confirmed by Sanger sequencing. pcDNA3-Myc-human HOIP and pcDNA3-human-HOIL-1L-HA were from Kazuhiro Iwai , pGex6P-1-human UbcH7, pET49b-human HOIP UBA-RBR-C (aa 476-1072) and pET49b-human HOIL-1L (C460A) were from Katrin Rittinger ( ). pOPINK-vOTU (CCHFV OTU, aa1-183) (Addgene plasmid #61589) ( ) and pOPINS-USP21 (USP, aa 196-565) (Addgene plasmid #61585)( ) were gifts from David Komander. ..

    Article Title: Linear ubiquitination by LUBEL has a role in Drosophila heat stress response.
    Article Snippet: For baculovirus-based insect expression, full-length LUBEL was subcloned into a modified version of the pACEBac1 vector (Geneva Biotech). .. Mutants including LUBEL-RBR-C (C2690S/C2693S), LUBEL-RBR-C (C2704S), LUBEL-RBR-C (R2745A), LUBEL-RBR-C (D2755A), dCYLD (C284S), and OTULIN (C129A) were generated by site-directed mutagenesis. pOPINK-vOTU (CCHFV OTU, aa1–183) was a gift from David Komander (Addgene plasmid # 61589) [28]. .. Human OTULIN cDNAcontaining plasmid was purchased from OriGene (Rockville, MD). pGex6P1-UbcH7 and pET49b-human HOIP-RBR-C were used elsewhere [30]. pcDNA3-Myc-human HOIP and pcDNA3-HA-human HOIL-1L were described previously [8,13].

    Expressing:

    Article Title: Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability
    Article Snippet: .. A plasmid expressing GST-Neh2Dual in bacteria under control of the T7 promoter was constructed by replacing vOTU from pOPINK-vOTU (Addgene plasmid # 61589, a gift from David Komander ) using Gibson cloning. ..

    Article Title: Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability
    Article Snippet: .. A plasmid expressing GST-Neh2Dual in bacteria under control of the T7 promoter was constructed by replacing vOTU from pOPINK-vOTU (Addgene plasmid # 61589, a gift from David Komander ) using Gibson cloning. ..

    Bacteria:

    Article Title: Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability
    Article Snippet: .. A plasmid expressing GST-Neh2Dual in bacteria under control of the T7 promoter was constructed by replacing vOTU from pOPINK-vOTU (Addgene plasmid # 61589, a gift from David Komander ) using Gibson cloning. ..

    Article Title: Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability
    Article Snippet: .. A plasmid expressing GST-Neh2Dual in bacteria under control of the T7 promoter was constructed by replacing vOTU from pOPINK-vOTU (Addgene plasmid # 61589, a gift from David Komander ) using Gibson cloning. ..

    Control:

    Article Title: Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability
    Article Snippet: .. A plasmid expressing GST-Neh2Dual in bacteria under control of the T7 promoter was constructed by replacing vOTU from pOPINK-vOTU (Addgene plasmid # 61589, a gift from David Komander ) using Gibson cloning. ..

    Article Title: Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability
    Article Snippet: .. A plasmid expressing GST-Neh2Dual in bacteria under control of the T7 promoter was constructed by replacing vOTU from pOPINK-vOTU (Addgene plasmid # 61589, a gift from David Komander ) using Gibson cloning. ..

    Construct:

    Article Title: Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability
    Article Snippet: .. A plasmid expressing GST-Neh2Dual in bacteria under control of the T7 promoter was constructed by replacing vOTU from pOPINK-vOTU (Addgene plasmid # 61589, a gift from David Komander ) using Gibson cloning. ..

    Article Title: Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability
    Article Snippet: .. A plasmid expressing GST-Neh2Dual in bacteria under control of the T7 promoter was constructed by replacing vOTU from pOPINK-vOTU (Addgene plasmid # 61589, a gift from David Komander ) using Gibson cloning. ..

    Cloning:

    Article Title: Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability
    Article Snippet: .. A plasmid expressing GST-Neh2Dual in bacteria under control of the T7 promoter was constructed by replacing vOTU from pOPINK-vOTU (Addgene plasmid # 61589, a gift from David Komander ) using Gibson cloning. ..

    Article Title: Ubiquitin receptors are required for substrate-mediated activation of the proteasome’s unfolding ability
    Article Snippet: .. A plasmid expressing GST-Neh2Dual in bacteria under control of the T7 promoter was constructed by replacing vOTU from pOPINK-vOTU (Addgene plasmid # 61589, a gift from David Komander ) using Gibson cloning. ..

    Generated:

    Article Title: Site-specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signaling.
    Article Snippet: .. Sequences of all the plasmids generated for this study were confirmed by Sanger sequencing. pcDNA3-Myc-human HOIP and pcDNA3-human-HOIL1L-HA were from Kazuhiro Iwai (Tokunaga et al, 2009), pGex6P1-human UbcH7 was from Katrin Rittinger (Stieglitz et al, 2012). pOPINK-vOTU (CCHFV OTU, aa1–183) (Addgene plasmid #61589) (Akutsu et al, 2011) and pOPINS-USP21 (USP, aa 196–565) (Addgene plasmid #61585) (Ye et al, 2011) were gifts from David Komander. ..

    Article Title: Site-specific ubiquitination of the E3 ligase HOIP regulates cell death and immune signaling
    Article Snippet: .. Sequences of all the plasmids generated for this study were confirmed by Sanger sequencing. pcDNA3-Myc-human HOIP and pcDNA3-human-HOIL-1L-HA were from Kazuhiro Iwai , pGex6P-1-human UbcH7, pET49b-human HOIP UBA-RBR-C (aa 476-1072) and pET49b-human HOIL-1L (C460A) were from Katrin Rittinger ( ). pOPINK-vOTU (CCHFV OTU, aa1-183) (Addgene plasmid #61589) ( ) and pOPINS-USP21 (USP, aa 196-565) (Addgene plasmid #61585)( ) were gifts from David Komander. ..

    Article Title: Linear ubiquitination by LUBEL has a role in Drosophila heat stress response.
    Article Snippet: For baculovirus-based insect expression, full-length LUBEL was subcloned into a modified version of the pACEBac1 vector (Geneva Biotech). .. Mutants including LUBEL-RBR-C (C2690S/C2693S), LUBEL-RBR-C (C2704S), LUBEL-RBR-C (R2745A), LUBEL-RBR-C (D2755A), dCYLD (C284S), and OTULIN (C129A) were generated by site-directed mutagenesis. pOPINK-vOTU (CCHFV OTU, aa1–183) was a gift from David Komander (Addgene plasmid # 61589) [28]. .. Human OTULIN cDNAcontaining plasmid was purchased from OriGene (Rockville, MD). pGex6P1-UbcH7 and pET49b-human HOIP-RBR-C were used elsewhere [30]. pcDNA3-Myc-human HOIP and pcDNA3-HA-human HOIL-1L were described previously [8,13].

    Sequencing:

    Article Title: Site-specific ubiquitination of the E3 ligase HOIP regulates apoptosis and immune signaling.
    Article Snippet: .. Sequences of all the plasmids generated for this study were confirmed by Sanger sequencing. pcDNA3-Myc-human HOIP and pcDNA3-human-HOIL1L-HA were from Kazuhiro Iwai (Tokunaga et al, 2009), pGex6P1-human UbcH7 was from Katrin Rittinger (Stieglitz et al, 2012). pOPINK-vOTU (CCHFV OTU, aa1–183) (Addgene plasmid #61589) (Akutsu et al, 2011) and pOPINS-USP21 (USP, aa 196–565) (Addgene plasmid #61585) (Ye et al, 2011) were gifts from David Komander. ..

    Article Title: Site-specific ubiquitination of the E3 ligase HOIP regulates cell death and immune signaling
    Article Snippet: .. Sequences of all the plasmids generated for this study were confirmed by Sanger sequencing. pcDNA3-Myc-human HOIP and pcDNA3-human-HOIL-1L-HA were from Kazuhiro Iwai , pGex6P-1-human UbcH7, pET49b-human HOIP UBA-RBR-C (aa 476-1072) and pET49b-human HOIL-1L (C460A) were from Katrin Rittinger ( ). pOPINK-vOTU (CCHFV OTU, aa1-183) (Addgene plasmid #61589) ( ) and pOPINS-USP21 (USP, aa 196-565) (Addgene plasmid #61585)( ) were gifts from David Komander. ..

    Mutagenesis:

    Article Title: Linear ubiquitination by LUBEL has a role in Drosophila heat stress response.
    Article Snippet: For baculovirus-based insect expression, full-length LUBEL was subcloned into a modified version of the pACEBac1 vector (Geneva Biotech). .. Mutants including LUBEL-RBR-C (C2690S/C2693S), LUBEL-RBR-C (C2704S), LUBEL-RBR-C (R2745A), LUBEL-RBR-C (D2755A), dCYLD (C284S), and OTULIN (C129A) were generated by site-directed mutagenesis. pOPINK-vOTU (CCHFV OTU, aa1–183) was a gift from David Komander (Addgene plasmid # 61589) [28]. .. Human OTULIN cDNAcontaining plasmid was purchased from OriGene (Rockville, MD). pGex6P1-UbcH7 and pET49b-human HOIP-RBR-C were used elsewhere [30]. pcDNA3-Myc-human HOIP and pcDNA3-HA-human HOIL-1L were described previously [8,13].



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